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SOD2 expression lentivirus (↑SOD2) significantly increased, while SOD2 knockdown lentivirus (shSOD2) significantly decreased, SOD2 mRNA levels (see Figure 1C)
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Counts were exported to MS Excel (Microsoft Corporation, Redmond, WA) and lethal fraction (LF) scores were computed from mKate2+ and SG+ counts, with the additional step of removing ‘overlap’ double positive counts from live cell counts at each timepoint, as described before (Forcina et al., 2017
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