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The activity of superoxide dismutase activity (SOD) (a), glutathione... | Download Scientific Diagram
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Comprehensive enzymatic tag-removal reviews highlight TEVโs advantages over other proteasesโincluding broad N-terminal residue tolerance, mild-temperature activity (including at 4 ยฐC), and robust cleavage efficiency, while also pointing out limitations such as occasional autolysis (that can be mitigated by S219V mutation in the TEV enzyme), reduced activity at higher temperatures, and the need for reducing agents in some constructs [139,161,162]
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