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Immunohistochemical (IHC) analysis of LCRMP-1 and microvessel density in tumor samples from experimental tumor growth in vivo for patients with NSCLC To prepare for IHC staining of LCRMP-1 and CD31 protein expression, the sections were first autoclaved in Antigen Retrieval Citra Solution (BioGenex) at 121 °C for 10 min
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A Waters Symmetry reverse-phase C18 column (2.1 mm × 150 mm, 3.5 μm) was used for the LC separation as described ( 8 and PGB 2 ] were monitored
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