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The loss of balance accompanied by trunk ataxia leads to the demand for patients to be wheelchair-bound by the third decade of the disease (Koeppen and Mazurkiewicz, 2013)
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The constructs CBS Δ516–525 , along with single point mutations of CBS, were amplified from the previously generated sequences, introduced into the pcDNA5-FRT backbone using In-Fusion (Takara) and confirmed by sequencing
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