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After calibration with 100 nm polystyrene beads, samples diluted in 0.22-ΞΌm-filtered PBS 1X (1:100) were injected using a 1 ml syringe
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3.1 Factors that determine cysteine availability Cysteine is derived normally from the diet, protein breakdown and in the liver, from methionine via transsulfuration (see below)
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