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(101) Cataract development in GGT1 -deficient mice appears to be a consequence of multiple causes, including exogenous damage (exposure to light), decreased lens glutathione levels, and nutritional effects of low cysteine availability
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For histone analysis, cell pellets were acid-extracted overnight using Abcam’s histone extraction protocol in phosphate-buffered saline containing 0.5% Triton X-100, 2 mM PMSF, 0.02% NaN 3 , and 10 mM sodium butyrate and supplemented with cOmplete Protease Inhibitor Cocktail before use
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