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To evaluate intracellular Ca 2+ levels during NET formation, 2 × 10 5 neutrophils labeled with Indo-1 were seeded in 96-well plates and induced by PMA (100 nM) in the presence or absence of agonists for 2 h at 37 °C in RPMI 1640

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A case-control study in Japan found that the greatest risk for aneurysmal SAH was in patients with a history of smoking and less frequent intake of antioxidant soy products [112]