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Various bioanalytical methods, such as chromatographic techniques for determining drug plasma concentrations and metabolism 569,572 and imaging technology for estimating drug distribution, 573 are used, although each has limitations

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102 , 103 A few studies have investigated the therapeutic efficacy of phlebotomy in patients with liver fibrosis, cirrhosis, and HCC, finding that liver function, hepatic fibrosis, and cirrhosis may be improved and reversed after long-term phlebotomy, while this data needs to be supported by randomized trials