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Gene Expression Analysis by RT-qPCR Purified RNA was treated with DNAse I, RNase-free (Thermo Scientific™, Waltham, MA, USA) according to the manufacturer’s protocol, and reverse transcribed into cDNA using the iScript™ Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, ON, Canada)
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The majority of the seaweed cell wall consists of over 40% of SP, which is substantially higher than the average found in other sources (134)
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