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the identification of the targeting event may also be facilitated by the use of one or more marker genes exhibiting the property of negative selection, such that the negatively selectable marker is linked to the exogenous DNA, but configured such that the negatively selectable marker flanks the targeting sequence, and such that a correct homologous recombination event with sequences in the host cell genome does not result in the stable integration of the negatively selectable marker

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It is commonly assumed to be linked to hyperglycemia and insulin resistance, the role of adipocytokines, irregular activation of RAAS, release of inflammatory factors, aberrant lipid metabolism, and renal structural damage caused by obesity itself (147)