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HRP-conjugated Protein A/G (1:5000) and SuperSignal West Pico PLUS substrate (Thermo Fisher Scientific) were used for detection and signals visualised using an Azure c280 imaging system (Cambridge Bioscience, Cambridge, UK)

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Finally, this review sums up with a discussion of how nitrogen homeostasis in the brain serves as a coordinating locus for the control of these nitrergic neurotransmitters, and approaches for identifying bona fide co-transmitter effects of these metabolites in future work