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Nine different substances were declared or identified as undeclared PCT drugs in the samples and, in order of occurrence frequency, they were clomiphene, hCG, tamoxifen, exemestane, anastrozole, cabergoline, letrozole, mesterolone, and raloxifene (Figure 9)

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The assay readout is generated by quantifying cells that reach the underside of the insert membrane or lower compartment after incubation, commonly by staining and counting invaded cells or by fluorescence-based quantification