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For instance, inhibiting Complex I of the mitochondrial electron transport chain reduces de novo l-serine biosynthesis from glucose in cancer cell lines, providing experimental evidence that links l-serine synthesis to mitochondrial function and NAD + /NADH balance (21, 79)

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This structural resistance to proteolysis allows GSH to accumulate intracellularly at millimolar concentrations (1-10 mM), making it the most abundant non-protein thiol in mammalian cells and the reference standard for intracellular antioxidant research

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