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In the cell, 90% of GSH is located in the cytoplasm, 10–12% in the mitochondria, and a small percentage in the endoplasmic reticulum (ER) ( The γ-glutamatyl cycle or Meister cycle initially proposed in the 60s described the synthesis and breakdown of GSH, making it a strong cysteine donor in physiological and pathological conditions ( de novo synthesis of GSH by cancer cells occurs as follows: GSH is first exported from the cell of origin via transporters known as Multiresistance Drug Proteins (MRPs), which belongs to the ATP binding cassette (ABC)s transporter family and is well-known player in cancer resistance mechanisms ( via the proton-coupled oligopeptide transporter family member PEPT2 (35, 36) or further cleaved by dipeptidases to cysteine and glycine

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Our data support the notion that C/EBPα was mechanistically involved in NMN-protected Hamp reduction and further hepatocyte lipid deposition based on the following evidence: (a) nuclear C/EBPα was significantly decreased in both alcohol-fed mice liver and ethanol-treated hepatocytes