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After the initial infection of the skin (stage 1), the spirochete often disseminates to many sites (stage 2), particularly to other skin sites, the nervous system, heart or joints, where it might cause persistent infection (stage 3) for years

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Cloning/lentivirus generation CRISPR–Cas9-mediated knockout of target genes was achieved by cloning sgRNAs into pLenti-USEC or lentiCRISPRv2-puro vectors, as previously described 31