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25,26 In order to inhibit the powerful antioxidant defense system of tumor cells, a small molecule L-Buthionine-sulfoximine (BSO) was utilized as an inhibitor of Ξ³-glutamylcysteine synthase (Ξ³-GCS), a key enzyme involved in GSH biosynthesis pathway, which could block the replenishment of GSH, leading to the inactivation of GPX4 and the accumulation of lipid peroxides
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