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For instance, LA was shown to cause cell-cycle blockade at the G 0 /G 1 and G 2 /M phases in colon carcinoma cells, alongside elevated reactive-oxygen-species production and glutathione depletion, thereby reducing cell viability (Figure 2) ( Cip1/WAF1 in a p53-independent manner, which contributes to growth arrest and reduced proliferation (Lappano et al., 2017)