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Affinity Chromatography and Importance in Drug Discovery | IntechOpen
Reaction was initiated by adding 120 μL of working solution (5,5’-dithiobis 2-nitrobenzoic acid and GR with 20 μL of cell extract (50 μg protein) or glutathione standards
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H 2 O 2 can be directly removed by the CAT enzyme, producing O 2 and H 2 O, while GPx uses H 2 O 2 and reduced glutathione GSH to form H 2 O and oxidized glutathione GSSG
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